Here is a run-down of those who will be missing and what impact it might have on Roberto Di Matteo’s side.
The captain’s needless first-half red card for clashing with Alexis Sanchez will deny him the chance of redemption for his penalty shoot-out miss in Moscow four years ago, a mis-kick which cost Chelsea their first chance at Champions League glory.
But putting his personal disappointment aside, the loss of their defensive leader will surely leave a hole at the back that Chelsea, especially if Cahill and Luiz are not fit in time.
The Serb defender, who played most of last night’s match as Chelsea’s only regular centre-half, talked himself into a suspension when he was booked for dissent for arguing over the award of a penalty for Didier Drogba’s foul on Cesc Fabregas.
The Brazilian midfielder found himself playing much of last night’s match at right-back in Chelsea’s reshuffled backline.
However, he still managed to get forward to score the dramatic 49th minute goal that would have been enough to send Chelsea through before Fernando Torres’ late strike made sure.
His audacious chip over Victor Valdes came after he was booked for dissent late in the first half, meaning he already knew he would not be part of the final he was helping Chelsea to reach.
The Portuguese midfielder will pay a heavy price for the 90th minute booking he received for a late sliding tackle on Javier Mascherano.
But the price Chelsea might pay remains to be seen as his absence alongside Ramires will rob Di Matteo of two of his more versatile midfield options.
Florent Malouda, Michael Essien and Salomon Kalou are the players that might stand to benefit, but all have suffered from either injuries or indifferent form and they are likely to leave Chelsea weaker in the middle of the park.